RHOBTB2:Rho-related BTB domain-containing protein 2

Gene Name
Rho-related BTB domain-containing protein 2
Protein ID
Chromosome ID
HPP Status
Protein Name
Rho-related BTB domain-containing protein 2

Rho-related BTB domain containing 2


deleted in breast cancer 2 gene protein

rho-related BTB domain-containing protein 2


Deleted in breast cancer 2 gene protein


[Reference: http://www.genecards.org/cgi-bin/carddisp.pl?gene=RHOBTB2 ]
Chromosomal Position
8p21.3 | Start:22987417 End:22987417

Entrez Gene Summary for RHOBTB2
The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Nov 2011)

[Reference: http://www.genecards.org/cgi-bin/carddisp.pl?gene=RHOBTB2 ]
External IDs
Hgnc ID: 18756 EntrezGene ID: 23221 Ensembl ID: ENSG00000008853
[Reference: http://www.genecards.org/cgi-bin/carddisp.pl?gene=RHOBTB2 ]
Reference Source

Gene Reference Into Function (GeneRIF)

PubMed IDGeneRIF TextLast Update
12370419A previously uncharacterized gene, DBC2 (deleted in breast cancer), was cloned from a homozygously deleted region at human chromosome 8p21.2010-01-21
15663929two networks were found to react substantially to DBC2 expression--one of the networks regulates cell growth through cell-cycle control and apoptosis; the other network is related to cytoskeleton and membrane trafficking2010-01-21
17023000DBC2 plays an essential role in microtubule-mediated VSVG transport from the endoplasmic reticulum to the Golgi apparatus.2010-01-21
17517369Our results indicate that the down-regulation of CCND1 is an essential step for DBC2's growth suppression of cancer cells.2010-01-21
17617377These findings indicate that the resistant T-47D cells survive DBC2 induction by rapid destruction of DBC2 through 26S proteasome-mediated protein degradation.2010-01-21
17653899The researchers isolated three novel mutations in the DBC2 gene in breast cancer families in Iran.2010-01-21
17906984Mutations & allelic loss in the DBC2 gene are uncommon in gastric cancers in Korean patients, so it is unlikely to play a major role. 3 new polymorphisms in the coding region were found.2010-01-21
18039672RhoBTB2 up-regulated during drug-induced apoptosis, with this being primarily dependent on E2F1.2010-01-21
18640857aberrant methylation in DBC2 promoter may be responsible for the expression loss of DBC2 expression in bladder cancer and this hypermethylation event could play a crucial role in the early stage of bladder tumorigenesis.2010-01-21
18762809CXCL14 is a gene target of RhoBTB2 and supports downregulation of CXCL14 as a functional outcome of RhoBTB2 loss in cancer.2010-01-21
19173804The expression of Fas, CTLA-4 and RhoBTB2 at the mRNA level is involved in genesis and progression of breast cancer.2010-05-10
19714462Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)2010-09-15
19773279Observational study of gene-disease association. (HuGE Navigator)2009-12-02
19937980Frequent loss of RhoBTB2 expression is associated with sporadic breast cancer.2010-03-29
20237496Observational study of gene-disease association. (HuGE Navigator)2010-04-07
20930524Ectopic expression of RhoBTB2 results in decreased phosphorylation of ezrin and Akt2 in both MDA-MB-231 and MDA-MB-435 cells.2011-07-02
20980811RhoBTB2 is establishing itself as a tumor suppressor with increasing relevance towards malignancies.2011-07-02
21801820Overexpression of RhoBTB2 in breast tumor cells significantly inhibited the proliferation and colony formation of tumor cells. In addition, RhoBTB2 also elevated the apoptotic ratio and caused typical changes of apoptotic morphology.2011-10-15
22901165Loss of DBC2 expression is an early and progressive event in the development of lung adenocarcinoma.2013-01-26
23546941Thus loss of DBC2 expression is caused by abnormal methylation of DBC2 and might have a role in breast cancer development.2014-02-01
24356943). The results demonstrated that aberrant methylation of RhoBTB2 may be responsible for the suppression of RhoBTB2 mRNA expression in breast cancer, a significant event during the genesis of breast cancer that correlated with progesterone receptor status2014-09-13
24485767Mutation in the promoter and exon 7 of DBC2 gene is not common in the Chinese population and may not contribute to the susceptibility for breast cancer in China2015-01-24
24608665data suggest a new paradigm for Hsp90-modulated assembly of a Cul3/DBC2 E3 ubiquitin ligase complex that may extend to other E3 ligase complexes.2015-01-03


Relevant citations within the PubMed literature


Putative/known Functions


Homologues, Orthologues, Paralogues and Family

Sequence Similarity and Functional Annotation

Sequence Similarity

Db NameQuery UniSubject UniSequence LengthAlignment LengthIdentityCoverageMismatchesGap OpeningsQuery StartQuery EndSubject StartSubject EndEvalueBit Score
Reviewed non-human mammalian with experimental evidenceRHBT2_HUMANRHBT1_MOUSE72771167.0997.82063170516890884
Islam MT, Garg G, Hancock WS, Risk BA, Baker MS, Ranganathan S (2014) Protannotator: A Semiautomated Pipeline for Chromosome-Wise Functional Annotation of the "Missing" Human Proteome. J Proteome Res. 13, 76-83.

InterProScan Annotation

Uniprot IDInterpro IDGo IDTypeNameCategoryDescription
RHBT2_HUMANIPR000210GO:0005515DomainBTB/POZ-likeMolecular Functionprotein binding
RHBT2_HUMANIPR001806GO:0005525FamilySmall GTPase superfamilyMolecular FunctionGTP binding
RHBT2_HUMANIPR001806GO:0007264FamilySmall GTPase superfamilyBiological Processsmall GTPase mediated signal transduction
RHBT2_HUMANIPR003578GO:0005525FamilySmall GTPase superfamily, Rho typeMolecular FunctionGTP binding
RHBT2_HUMANIPR003578GO:0005622FamilySmall GTPase superfamily, Rho typeCellular Componentintracellular
RHBT2_HUMANIPR003578GO:0007264FamilySmall GTPase superfamily, Rho typeBiological Processsmall GTPase mediated signal transduction
RHBT2_HUMANIPR003579GO:0005525FamilySmall GTPase superfamily, Rab typeMolecular FunctionGTP binding
RHBT2_HUMANIPR003579GO:0007264FamilySmall GTPase superfamily, Rab typeBiological Processsmall GTPase mediated signal transduction
RHBT2_HUMANIPR003579GO:0015031FamilySmall GTPase superfamily, Rab typeBiological Processprotein transport
RHBT2_HUMANIPR013069GO:0005515DomainBTB/POZMolecular Functionprotein binding
RHBT2_HUMANIPR020849GO:0003924FamilySmall GTPase superfamily, Ras typeMolecular FunctionGTPase activity
RHBT2_HUMANIPR020849GO:0005525FamilySmall GTPase superfamily, Ras typeMolecular FunctionGTP binding
RHBT2_HUMANIPR020849GO:0006184FamilySmall GTPase superfamily, Ras typeBiological ProcessGTP catabolic process
RHBT2_HUMANIPR020849GO:0007165FamilySmall GTPase superfamily, Ras typeBiological Processsignal transduction
RHBT2_HUMANIPR020849GO:0016020FamilySmall GTPase superfamily, Ras typeCellular Componentmembrane
Islam MT, Garg G, Hancock WS, Risk BA, Baker MS, Ranganathan S (2014) Protannotator: A Semiautomated Pipeline for Chromosome-Wise Functional Annotation of the "Missing" Human Proteome. J Proteome Res. 13, 76-83.

KEGG Pathways

Pathway IDDatabase NamePathway Name
hsa04120KEGG PATHWAYUbiquitin mediated proteolysis
Islam MT, Garg G, Hancock WS, Risk BA, Baker MS, Ranganathan S (2014) Protannotator: A Semiautomated Pipeline for Chromosome-Wise Functional Annotation of the "Missing" Human Proteome. J Proteome Res. 13, 76-83.

Post Translational Modifications

Protein Protein Interactions

Best Available Mass Spectra without FDR


Evidence File
Fenyö, David; Beavis, Ronald C. (2015). "The GPMDB REST interface". Bioinformatics 31 (12): 2056–2058. doi:10.1093/bioinformatics/btv107. ISSN 1367-4803.
http://gpmdb.thegpm.org/ ">Global Proteome Machine Database - THE GPM


Peptide SequenceScoresPride IDSpectrum IDAnnotation
No results found.
Evidence File
Vizcaino JA, et al. 2016 update of the PRIDE database and related tools. Nucleic Acids Res. 2016 Jan 1;44(D1):D447-D456.
https://www.ebi.ac.uk/pride/archive/ ">PRIDE Archive

Proteomics DB

Evidence File
Wilhelm, M et al. (2014) Mass-spectrometry-based draft of the human proteome. Nature. 509:582-7.
https://www.proteomicsdb.org/ ">Proteomics DB